Biological assay for Clostridium perfringens enterotoxin with vero cells.
نویسندگان
چکیده
منابع مشابه
Vero cell assay for rapid detection of Clostridium perfringens enterotoxin.
A rapid assay which measured the biological activity of Clostridium perfringens enterotoxin was developed. The method involved the rapid killing of Vero cells by enterotoxin produced by C. perfringens grown in Duncan and Strong sporulation medium. Serial dilutions of toxin were added to Vero cells either in suspension or grown as monolayers in wells of a 96-well cell tissue culture cluster plat...
متن کاملAssay methods for Clostridium perfringens type A enterotoxin.
Enterotoxin produced by a sporulating culture of Clostridium perfringens type A NCTC 8798 was purified to a level of 3,500 mouse mean lethal doses per mg of nitrogen. High-titer sera were obtained from rabbits injected with enterotoxin and used to compare the sensitivity of serological tests and bioassays for C. perfringens enterotoxin. Reversed passive hemagglutination was by far the most sens...
متن کاملUse of dogs as an assay for Clostridium perfringens enterotoxin.
Three techniques for using the dog as an assay organism for Clostridium perfringens enterotoxin are described. These are believed to be more convenient than ligated ileal-loop procedures.
متن کاملEvaluation of ELISA, RPLA, and Vero cell assays for detecting Clostridium perfringens enterotoxin in faecal specimens.
Three hundred and ninety two faecal specimens from 70 separate outbreaks of suspected Clostridium perfringens food poisoning were examined by enzyme linked immunosorbent assay (ELISA), reversed passive latex agglutination (RPLA), and Vero cell assays for the presence of enterotoxin. Although the most time consuming method, ELISA was the most specific and reproducible. RPLA was slightly more sen...
متن کاملClostridium perfringens enterotoxin: a brief review.
Ussing chambers were mounted with stripped rabbit ileal mucosa and either crude or pure toxin A added to the mucosal side of the chamber to final concentrations of 800 and 30pg/ml respectively; increases in short-circuit currents of the order of 25pA were observed in both preparations over a period of 90min, at which point the tissue was reponsive to theophylline but not glucose. Histology show...
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ژورنال
عنوان ژورنال: The Japanese Journal of Veterinary Science
سال: 1984
ISSN: 0021-5295,1881-1442
DOI: 10.1292/jvms1939.46.715